7S0J

Crystal structure of Epstein-Barr virus gH/gL targeting antibody 769B10

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-10-02
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.987
Spacegroup name	P 1 21 1
Unit cell lengths	56.174, 67.051, 65.030
Unit cell angles	90.00, 107.64, 90.00

Refinement procedure

Resolution	41.830 - 2.150
R-factor	0.2366
Rwork	0.235
R-free	0.26190
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4uv4
RMSD bond length	0.002
RMSD bond angle	0.579
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.250
High resolution limit [Å]	2.150	2.150
Number of reflections	28030	22877
<I/σ(I)>	5.3
Completeness [%]	89.7
Redundancy	4.5
CC(1/2)	0.820	0.720

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1M Tris-HCl pH8.0, 18% PEG5000MME, 0.2M NaCl, 2.5% Glycerol