7RX9

Structure of autoinhibited P-Rex1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-07-07
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.95373
Spacegroup name	P 41 2 2
Unit cell lengths	151.256, 151.256, 94.287
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.830 - 3.220
R-factor	0.2375
Rwork	0.236
R-free	0.26210
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4yon
RMSD bond length	0.003
RMSD bond angle	0.544
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.830	3.480
High resolution limit [Å]	3.220	3.220
Number of reflections	18039	3597
<I/σ(I)>	7.8
Completeness [%]	99.1
Redundancy	6.4
CC(1/2)	0.998	0.358

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	1.8 M (NH4)2SO4, 0.05 MES pH 6.0