7RGF

Protocadherin gammaC4 EC1-4 crystal structure disrupted trans interface

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-03-04
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	51.944, 103.948, 200.592
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.160 - 2.400
R-factor	0.1977
Rwork	0.196
R-free	0.24050
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7jgz
RMSD bond length	0.003
RMSD bond angle	0.653
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.490
High resolution limit [Å]	2.400	2.400
Rmerge	0.060	0.438
Rmeas	0.073	0.560
Rpim	0.040	0.342
Number of reflections	42979	4175
<I/σ(I)>	13.3	2.5
Completeness [%]	98.9	92.6
Redundancy	5.6	4
CC(1/2)	0.999	0.924

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE		293	1 M LiCl, 0.1 M Mes pH 6.0, and 10% (w/v) PEG6000