7RE7
TCR mimic antibody (Fab fragment) in complex with AFP/HLA-A*02
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-11-25 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.97918 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 49.707, 200.912, 244.574 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.201 - 2.547 |
R-factor | 0.1837 |
Rwork | 0.180 |
R-free | 0.21960 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5e00 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.580 |
High resolution limit [Å] | 2.540 | 2.540 |
Number of reflections | 81215 | 3257 |
<I/σ(I)> | 19.9 | |
Completeness [%] | 99.3 | |
Redundancy | 12.4 | |
CC(1/2) | 0.994 | 0.764 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 296.15 | 18% v/v PEG 3350 and 0.2 M sodium formate solution, pH 7.2 |