7RDW
Crystal Structure of FH1 Fab bound to HXb2 HIV-1 gp120 core
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-11-01 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 |
| Unit cell lengths | 103.361, 106.266, 113.219 |
| Unit cell angles | 67.57, 76.67, 60.99 |
Refinement procedure
| Resolution | 50.180 - 3.550 |
| R-factor | 0.2619 |
| Rwork | 0.260 |
| R-free | 0.29490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6mft |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.622 |
| Data reduction software | HKL-2000 (1.15.2_3472) |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.180 | 3.560 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Number of reflections | 36171 | 1577 |
| <I/σ(I)> | 5.29 | |
| Completeness [%] | 92.5 | 69.3 |
| Redundancy | 1.6 | |
| CC(1/2) | 0.950 | 0.712 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M NH4SO4, 20% PEG 1500, 0.1M Tris pH 7.5 |
