7RDW
Crystal Structure of FH1 Fab bound to HXb2 HIV-1 gp120 core
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-11-01 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1 |
Spacegroup name | P 1 |
Unit cell lengths | 103.361, 106.266, 113.219 |
Unit cell angles | 67.57, 76.67, 60.99 |
Refinement procedure
Resolution | 50.180 - 3.550 |
R-factor | 0.2619 |
Rwork | 0.260 |
R-free | 0.29490 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6mft |
RMSD bond length | 0.008 |
RMSD bond angle | 1.622 |
Data reduction software | HKL-2000 (1.15.2_3472) |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.180 | 3.560 |
High resolution limit [Å] | 3.500 | 3.500 |
Number of reflections | 36171 | 1577 |
<I/σ(I)> | 5.29 | |
Completeness [%] | 92.5 | 69.3 |
Redundancy | 1.6 | |
CC(1/2) | 0.950 | 0.712 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M NH4SO4, 20% PEG 1500, 0.1M Tris pH 7.5 |