7RDT
Structure of human NTHL1 - linker 1 chimera
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-10-20 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.0332 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 42.370, 71.680, 86.520 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.050 - 2.100 |
R-factor | 0.1861 |
Rwork | 0.181 |
R-free | 0.23060 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7rds |
RMSD bond length | 0.002 |
RMSD bond angle | 0.471 |
Data reduction software | MOSFLM |
Data scaling software | pointless |
Phasing software | PHENIX |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.050 | 2.160 |
High resolution limit [Å] | 2.100 | 2.100 |
Rpim | 0.053 | 0.210 |
Number of reflections | 28900 | 1269 |
<I/σ(I)> | 8.9 | 3.4 |
Completeness [%] | 100.0 | 99.5 |
Redundancy | 4.1 | 4 |
CC(1/2) | 0.995 | 0.843 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.3 | 291 | PEG 6K, Sodium chloride, Tricine, |