7RAF

AncAR1-Rev - progesterone - Tif2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	80
Detector technology	CCD
Collection date	2016-01-04
Detector	MAR CCD 130 mm
Wavelength(s)	1
Spacegroup name	P 43 21 2
Unit cell lengths	69.099, 69.099, 145.258
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.612 - 1.551
R-factor	0.1536
Rwork	0.153
R-free	0.16880
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ama
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (v1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.700	10.000
High resolution limit [Å]	1.500	1.500
Number of reflections	51730	51730
<I/σ(I)>	31.6
Completeness [%]	99.8
Redundancy	31.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		280	25% PEG8000 and 50mM Tris pH 8.5