7R5W

Crystal structure of YTHDF2 with compound YLI_DF_029

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-12-01
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1
Spacegroup name	P 65
Unit cell lengths	81.060, 81.060, 113.360
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.100 - 1.750
R-factor	0.199
Rwork	0.198
R-free	0.22590
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4rdn
RMSD bond length	0.007
RMSD bond angle	0.930
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.100	1.850
High resolution limit [Å]	1.750	1.750
Number of reflections	42878	6872
<I/σ(I)>	31.92
Completeness [%]	99.9
Redundancy	12.65
CC(1/2)	0.999	0.981

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277.15	1.975 M Ammonium sulfate, 0.1 M Sodium citrate tribasic pH 5.0