7R58

Crystal structure of the GPVI-glenzocimab complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-03-22
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	68.669, 93.195, 110.420
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.430 - 1.902
R-factor	0.1885
Rwork	0.186
R-free	0.23190
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5aze 3gwk 5ou7
RMSD bond length	0.008
RMSD bond angle	1.000
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	BUSTER (2.11.8)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.430	1.950
High resolution limit [Å]	1.900	1.900
Rmeas	0.265	1.851
Rpim	0.072	0.512
Number of reflections	56379	3695
<I/σ(I)>	9.9	1.6
Completeness [%]	100.0
Redundancy	13.2
CC(1/2)	0.995	0.598

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293.15	20 % PEG 3350 0.2 M KSCN 0.1 M Bis-Tris propane pH 8.5