7R2V
Structure of nsp14 from SARS-CoV-2 in complex with SAH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X6A |
| Synchrotron site | NSLS |
| Beamline | X6A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-02-01 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 67.176, 100.312, 90.874 |
| Unit cell angles | 90.00, 107.66, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.530 |
| R-factor | 0.1991 |
| Rwork | 0.196 |
| R-free | 0.25430 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7qgi |
| RMSD bond length | 0.003 |
| RMSD bond angle | 1.254 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 86.600 | 2.885 |
| High resolution limit [Å] | 2.530 | 2.532 |
| Rmerge | 0.160 | 0.914 |
| Number of reflections | 19046 | 2830 |
| <I/σ(I)> | 6.8 | |
| Completeness [%] | 87.8 | |
| Redundancy | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 278 | PEG/Imidazole |
