7QUH

Siglec-8 in complex with therapeutic Fab AK002.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-04-29
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.00003
Spacegroup name	C 1 2 1
Unit cell lengths	145.343, 44.554, 111.387
Unit cell angles	90.00, 111.41, 90.00

Refinement procedure

Resolution	42.319 - 2.867
R-factor	0.2277
Rwork	0.225
R-free	0.28360
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7qu6
Data reduction software	autoPROC
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.320	2.970
High resolution limit [Å]	2.867	2.870
Rmerge	0.233	1.042
Rpim	0.097	0.446
Number of reflections	14935	1575
<I/σ(I)>	9.3
Completeness [%]	95.4
Redundancy	6.7
CC(1/2)	0.985	0.678

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.2M ammonium chloride 20% PEG3350 pH6.3