7QPI

Structure of lamprey VDR in complex with 1,25D3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-07-24
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.98
Spacegroup name	P 21 21 21
Unit cell lengths	37.320, 66.170, 105.690
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.760 - 2.500
R-factor	0.1863
Rwork	0.182
R-free	0.25590
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2hc4
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.17_3644)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.290	2.584
High resolution limit [Å]	2.495	2.495
Number of reflections	9594	855
<I/σ(I)>	8.76
Completeness [%]	99.3
Redundancy	2
CC(1/2)	0.989	0.694

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	PEG 3350 25%, Tris 0.1M pH 8.5, magnesium chloride 0.2M