7QNW

The receptor binding domain of SARS-CoV-2 Omicron variant spike glycoprotein in complex with Beta-55 and EY6A Fabs

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-12-15
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.9763
Spacegroup name	P 32 2 1
Unit cell lengths	131.966, 131.966, 117.338
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	114.290 - 2.400
R-factor	0.2077
Rwork	0.206
R-free	0.24740
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7nx8
RMSD bond length	0.002
RMSD bond angle	0.492
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX (1.19_4092)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	117.000	2.440
High resolution limit [Å]	2.400	2.400
Rmerge	0.339
Rpim	0.053	0.458
Number of reflections	46542	2302
<I/σ(I)>	8.5	0.7
Completeness [%]	100.0
Redundancy	41.1
CC(1/2)	0.997	0.319

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M (NH4)2COOH, 0.1 M Hepes, pH 7.5 and 25% (w/v) PEG 3350