7QIF

Crystal structure of SARS-CoV-2 NSP14 in complex with 7MeGpppG.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-12-10
Detector	DECTRIS EIGER R 4M
Wavelength(s)	0.97625
Spacegroup name	P 21 21 21
Unit cell lengths	67.336, 67.314, 138.585
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	69.290 - 2.530
R-factor	0.211
Rwork	0.207
R-free	0.24650
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7qgi
RMSD bond length	0.002
RMSD bond angle	0.466
Data reduction software	DIALS
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	69.300	2.730
High resolution limit [Å]	2.530	2.530
Rmerge	0.585
Number of reflections	12041	602
<I/σ(I)>	6	1.6
Completeness [%]	91.7	65.9
Redundancy	26.5	27.6
CC(1/2)	0.986	0.747

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277.15	1.26M sodium phosphate monobasic, 0.14M potassium phosphate dibasic