7QGS

Crystal structure of p300 CH1 domain in complex with CITIF (a CITED2-HIF-1alpha hybrid)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-02-17
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	1.28367
Spacegroup name	P 1 21 1
Unit cell lengths	30.505, 48.599, 39.825
Unit cell angles	90.00, 103.87, 90.00

Refinement procedure

Resolution	38.660 - 2.000
R-factor	0.2221
Rwork	0.221
R-free	0.24500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7lvs
RMSD bond length	0.016
RMSD bond angle	1.545
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.660	2.030
High resolution limit [Å]	2.000	2.000
Rmerge	0.109	0.744
Rmeas	0.119	0.814
Rpim	0.047	0.325
Number of reflections	7708	355
<I/σ(I)>	7.5	2.9
Completeness [%]	99.7	96.5
Redundancy	6.6	6
CC(1/2)	0.991	0.870

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	0.1 M HEPES pH 6.5, PEG 6K 35%