7Q9B
MHC Class I A02 Allele presenting EAAGIGILTV, in complex with Mel8 TCR
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-01-17 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 0.97625 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 99.972, 53.645, 203.507 |
| Unit cell angles | 90.00, 94.33, 90.00 |
Refinement procedure
| Resolution | 99.686 - 3.240 |
| Rwork | 0.226 |
| R-free | 0.27650 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3hg1 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.452 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.700 | 3.320 |
| High resolution limit [Å] | 3.240 | 3.240 |
| Rmerge | 0.280 | 0.949 |
| Rmeas | 0.328 | 1.102 |
| Rpim | 0.220 | 0.719 |
| Number of reflections | 34992 | 2579 |
| <I/σ(I)> | 2.7 | |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 3.7 | 3.8 |
| CC(1/2) | 0.995 | 0.686 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 |
