7Q5W

The tandem SH2 domains of SYK with a bound TYROBP diphospho-ITAM peptide

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-06-27
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.9763
Spacegroup name	P 21 21 21
Unit cell lengths	117.949, 131.928, 141.526
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	65.960 - 2.200
Rwork	0.234
R-free	0.26630
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1a81
RMSD bond length	0.007
RMSD bond angle	1.420
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	65.960	2.240
High resolution limit [Å]	2.200	2.200
Rmerge	0.132	4.191
Rmeas	0.137	4.259
Rpim	0.039	1.195
Number of reflections	112454	5513
<I/σ(I)>	14	0.8
Completeness [%]	100.0	100
Redundancy	24.2	25.8
CC(1/2)	0.999	0.571

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	30 mM sodium nitrate, 30 mM dibasic sodium phosphate, 30 mM ammonium sulphate, 100 mM Tris/BICINE, 10% PEG 20,000, 20% PEG 500 MME