7Q4Q
Magacizumab Fab fragment in complex with human LRG1 epitope
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-11-28 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 64.549, 76.978, 217.731 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.460 - 1.650 |
| R-factor | 0.1842 |
| Rwork | 0.183 |
| R-free | 0.20680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5dk3 6al4 6bjz |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.902 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.460 | 1.680 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.075 | 1.786 |
| Rpim | 0.021 | 0.500 |
| Number of reflections | 131287 | 6412 |
| <I/σ(I)> | 18.3 | 1.6 |
| Completeness [%] | 100.0 | |
| Redundancy | 13.1 | |
| CC(1/2) | 0.990 | 0.632 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 287 | 0.2 M NaCl, 0.1 M Bis-Tris pH 5.5, 25% (w/v) PEG 3350 |
