7Q3P

Crystal structure of IgG1-Fc-MST-HN (efgartigimod)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-08-25
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9763
Spacegroup name	C 1 2 1
Unit cell lengths	96.408, 87.935, 106.093
Unit cell angles	90.00, 114.04, 90.00

Refinement procedure

Resolution	48.140 - 2.100
R-factor	0.19
Rwork	0.189
R-free	0.21390
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4n0u
RMSD bond length	0.009
RMSD bond angle	1.331
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.140	2.172
High resolution limit [Å]	2.097	2.097
Number of reflections	46871	4414
<I/σ(I)>	12.42	1.09
Completeness [%]	98.8	93.89
Redundancy	7	6.9
CC(1/2)	0.998	0.464

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.1	294	20% w/v PEG3350 pH 5.1. Cyroprotectant: mother liquid + 20% ethylene glycol (final concentration)