7Q2J
Quaternary Complex of human WDR5 and pVHL:ElonginC:ElonginB bound to PROTAC Homer
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-12-18 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.999998 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 47.702, 190.809, 48.698 |
Unit cell angles | 90.00, 115.25, 90.00 |
Refinement procedure
Resolution | 47.750 - 2.500 |
R-factor | 0.2459 |
Rwork | 0.243 |
R-free | 0.29320 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2h9m |
RMSD bond length | 0.005 |
RMSD bond angle | 1.429 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.750 | 2.600 |
High resolution limit [Å] | 2.500 | 2.500 |
Number of reflections | 26976 | 3045 |
<I/σ(I)> | 8.8 | |
Completeness [%] | 99.3 | |
Redundancy | 7.1 | |
CC(1/2) | 0.997 | 0.786 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.8 | 293 | 21% PEG 3350 0.4 M KSCN 0.1 M HEPES 6.8 |