7PO6

Xist (m6A)UCG tetraloop RNA bound to the YTH domain of YTHDC1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-10-11
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	78.438, 85.119, 88.617
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.340 - 1.770
R-factor	0.2017
Rwork	0.200
R-free	0.23220
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4r3i
RMSD bond length	0.011
RMSD bond angle	1.317
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.340	2.000
High resolution limit [Å]	1.770	1.870
Number of reflections	57964	17057
<I/σ(I)>	10.08	1.46
Completeness [%]	98.7
Redundancy	6.3
CC(1/2)	0.998	0.720

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	294	0.1M Tris HCl, 0.2M lithium sulfate, 30% (w/v) PEG 4000