7PHE
Chimeric carminomycin-4-O-methyltransferase (DnrK) with regions from 10-hydroxylase RdmB and 10-decarboxylase TamK
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX IV BEAMLINE BioMAX |
| Synchrotron site | MAX IV |
| Beamline | BioMAX |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-04-11 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 58.800, 110.400, 64.210 |
| Unit cell angles | 90.00, 108.65, 90.00 |
Refinement procedure
| Resolution | 55.710 - 2.320 |
| R-factor | 0.2148 |
| Rwork | 0.213 |
| R-free | 0.25470 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1tw2 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.647 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.713 | 2.356 |
| High resolution limit [Å] | 2.316 | 2.316 |
| Rpim | 0.124 | 0.537 |
| Number of reflections | 33595 | 1674 |
| <I/σ(I)> | 8.7 | |
| Completeness [%] | 99.4 | |
| Redundancy | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293.15 | 0.2 M Magnesium chloride hexahydrate; 0.1 M HEPES 7.0; 20 % w/v PEG 6000 |
