7PGJ
Chimeric carminomycin-4-O-methyltransferase (DnrK) with regions from 10-decarboxylate TamK and 10-hydroxylase RdmB, together with a single point mutation F297G
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE MASSIF-3 |
Synchrotron site | ESRF |
Beamline | MASSIF-3 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-10-01 |
Detector | DECTRIS EIGER X 4M |
Wavelength(s) | 0.987 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 60.750, 124.950, 102.340 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 53.320 - 2.130 |
R-factor | 0.1682 |
Rwork | 0.166 |
R-free | 0.21720 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1tw2 |
RMSD bond length | 0.008 |
RMSD bond angle | 0.922 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 62.500 | 2.210 |
High resolution limit [Å] | 2.130 | 2.130 |
Rmerge | 0.067 | 0.184 |
Number of reflections | 22162 | 2165 |
<I/σ(I)> | 22.4 | 13.4 |
Completeness [%] | 99.9 | 100 |
Redundancy | 6.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293.15 | in 0.1 M Sodium acetate, pH 4.6; 8% PEG 8000 |