7PGJ

Chimeric carminomycin-4-O-methyltransferase (DnrK) with regions from 10-decarboxylate TamK and 10-hydroxylase RdmB, together with a single point mutation F297G

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Carminomycin 4-O-methyltransferase DnrK,Methyltransferase domain-containing protein,Aclacinomycin 10-hydroxylase RdmB	polymer	368	39910.3	1	UniProt (Q06528) UniProt (I2N5E8) UniProt (Q54527) Pfam (PF00891) In PDB	Streptomyces peucetius	COMT,Anthracycline 4-O-methyltransferase,O-methyltransferase,15-demethoxy-epsilon-rhodomycin 10-hydroxylase,15-demethoxyaclacinomycin T
2	A	S-ADENOSYL-L-HOMOCYSTEINE	non-polymer		384.4	1	Chemie (SAH)
3	A	methyl (1R,2R,4S)-2-ethyl-7-methoxy-2,4,5-tris(oxidanyl)-6,11-bis(oxidanylidene)-3,4-dihydro-1H-tetracene-1-carboxylate	non-polymer		426.4	1	Chemie (ZBX)
4		water	water		18.0	261	Chemie (HOH)

Sequence modifications

A: 2 - 159 (UniProt: Q06528)

PDB	External Database	Details
Met -9	-	initiating methionine
Ala -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
Arg 0	-	expression tag
Ser 1	-	expression tag

A: 160 - 169 (UniProt: I2N5E8)
A: 170 - 285 (UniProt: Q06528)
A: 286 - 302 (UniProt: Q54527)

PDB	External Database	Details
Gly 299	Phe 300	engineered mutation

A: 303 - 336 (UniProt: Q06528)
A: 337 - 349 (UniProt: I2N5E8)
A: 350 - 360 (UniProt: Q06528)

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	39910.3
Non-Polymers*	Number of molecules	2
	Total formula weight	810.8
All*	Total formula weight	40721.2

*Water molecules are not included.