7P8Q

Structure of E.coli RlmJ in complex with an RNA conjugate (GA-SAM)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-03-17
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.978
Spacegroup name	C 1 2 1
Unit cell lengths	80.320, 38.300, 90.800
Unit cell angles	90.00, 105.36, 90.00

Refinement procedure

Resolution	39.500 - 2.289
R-factor	0.2206
Rwork	0.218
R-free	0.27540
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6qdx
Data reduction software	XDS
Data scaling software	Aimless (0.7.4)
Phasing software	MOLREP
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.500	2.370
High resolution limit [Å]	2.289	2.289
Rmerge	0.176	0.759
Rmeas	0.194	0.827
Rpim	0.079	0.325
Number of reflections	12282	1108
<I/σ(I)>	7.59
Completeness [%]	98.8	89.94
Redundancy	6	6.4
CC(1/2)	0.989	0.809

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7	293	0.1M BIS-TRIS pH 6.5 0.2M NaCl 25% w/v PEG 3350