7OY1
DnrK mutant RTCR
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MAX IV BEAMLINE BioMAX |
Synchrotron site | MAX IV |
Beamline | BioMAX |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-10-05 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.987 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 60.400, 105.690, 64.450 |
Unit cell angles | 90.00, 111.18, 90.00 |
Refinement procedure
Resolution | 49.700 - 2.390 |
R-factor | 0.2293 |
Rwork | 0.227 |
R-free | 0.26680 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1tw2 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.179 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.710 | 2.480 |
High resolution limit [Å] | 2.390 | 2.390 |
Rmerge | 0.242 | |
Rmeas | 0.138 | |
Rpim | 0.169 | |
Number of reflections | 29636 | 2854 |
<I/σ(I)> | 4.1 | |
Completeness [%] | 99.1 | |
Redundancy | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 0.2 M Magnesium chloride hexahydrate; 0.1 M HEPES 7.0; 20 % w/v PEG 6000 |