7OY1

DnrK mutant RTCR

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Carminomycin 4-O-methyltransferase DnrK,Methyltransferase domain-containing protein,Aclacinomycin 10-hydroxylase RdmB	polymer	368	39949.2	2	UniProt (Q06528) UniProt (A0A2V2Q0Q4) UniProt (Q54527) UniProt (I2N5E8) Pfam (PF00891)	Streptomyces peucetius	COMT,Anthracycline 4-O-methyltransferase,15-demethoxy-epsilon-rhodomycin 10-hydroxylase,15-demethoxyaclacinomycin T
2	C, E (A, B)	S-ADENOSYL-L-HOMOCYSTEINE	non-polymer		384.4	2	Chemie (SAH)
3	D, F (A, B)	methyl (1R,2R,4S)-2-ethyl-2,5,7-trihydroxy-6,11-dioxo-4-{[2,3,6-trideoxy-3-(dimethylamino)-alpha-L-lyxo-hexopyranosyl]oxy}-1,2,3,4,6,11-hexahydrotetracene-1-carboxylate	non-polymer		569.6	2	Chemie (3VL)
4	G, H (A, B)	water	water		18.0	264	Chemie (HOH)

Sequence modifications

A, B: 2 - 159 (UniProt: Q06528)

PDB	External Database	Details
Met -9	-	initiating methionine
Ala -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
Arg 0	-	expression tag
Ser 1	-	expression tag

A, B: 160 - 169 (UniProt: A0A2V2Q0Q4)
A, B: 170 - 184 (UniProt: Q06528)
A, B: 185 - 191 (UniProt: Q54527)
A, B: 192 - 285 (UniProt: Q06528)
A, B: 286 - 300 (UniProt: Q54527)
A, B: 301 - 334 (UniProt: Q06528)
A, B: 335 - 347 (UniProt: I2N5E8)

PDB	External Database	Details
Ser 347	Cys 358	engineered mutation

A, B: 348 - 358 (UniProt: Q06528)

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	79898.4
Non-Polymers*	Number of molecules	4
	Total formula weight	1908.0
All*	Total formula weight	81806.4

*Water molecules are not included.