7OX4

Mouse interleukin-9 in complex with Fab 35D8.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-04-28
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9762
Spacegroup name	C 2 2 21
Unit cell lengths	70.210, 78.920, 192.100
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.020 - 1.800
R-factor	0.1857
Rwork	0.183
R-free	0.22880
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4zs7 5bv7
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.020	1.910
High resolution limit [Å]	1.800	1.800
Number of reflections	49669	7855
<I/σ(I)>	9.49
Completeness [%]	99.7
Redundancy	5.8
CC(1/2)	0.997	0.443

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2M Zinc acetate, 23% PEG 3350