7OX2

Fab 6E2: hIL-9 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-01-29
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97242
Spacegroup name	P 1
Unit cell lengths	69.358, 94.364, 107.331
Unit cell angles	82.54, 72.00, 80.79

Refinement procedure

Resolution	48.920 - 3.340
R-factor	0.2268
Rwork	0.224
R-free	0.28350
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6ghg 5y9j
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.920	3.540
High resolution limit [Å]	3.340	3.340
Number of reflections	34638	5336
<I/σ(I)>	4.78
Completeness [%]	93.6
Redundancy	2.9
CC(1/2)	0.960	0.517

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	3.8	293	0.2M lithium sulfate, 0.1M phosphate/ citric acid, pH 3.8