7OUV

Crystal structure of human filamin C domains 14-15 cardiovascular disease causing mutation S1624L

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-02-01
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	39.133, 70.498, 156.937
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.230 - 1.800
R-factor	0.2136
Rwork	0.213
R-free	0.24020
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	unreleased
RMSD bond length	0.004
RMSD bond angle	0.675
Data reduction software	XDS
Data scaling software	XDS (1.19.2_4158)
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.230	1.864
High resolution limit [Å]	1.800	1.800
Rmerge	0.147	2.913
Rmeas	0.153	3.027
Rpim	0.044	0.816
Number of reflections	41232	4057
<I/σ(I)>	9.99	0.76
Completeness [%]	99.6	99.8
Redundancy	12.6
CC(1/2)	0.998	0.478

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	295.15	0.1 M SPG pH 5.0, 25% w/v PEG 1500