7OPQ

Rab27a fusion with Slp2a-RBDa1 effector covalent adduct with CA1 in C188

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-05-30
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	61.710, 76.816, 117.818
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.520 - 2.230
R-factor	0.1794
Rwork	0.177
R-free	0.22390
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3bc1
Data reduction software	DIALS
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX (1.17_3644)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.520	2.310
High resolution limit [Å]	2.230	2.230
Rmerge	0.034	0.360
Rmeas	0.048	0.509
Rpim	0.034	0.360
Number of reflections	54026	2005
<I/σ(I)>	13.67	2.01
Completeness [%]	96.9	72.64
Redundancy	2	2
CC(1/2)	0.998	0.760

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	277.15	0.15M Ammonium sulphate, 0.1M MES pH 6.0, 15% w/v PEG 4000