7OPM
Phosphorylated ERK2 in complex with ORF45
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P14 (MX2) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-11-13 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9762 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 210.094, 41.149, 51.701 |
Unit cell angles | 90.00, 92.35, 90.00 |
Refinement procedure
Resolution | 47.120 - 2.450 |
R-factor | 0.237 |
Rwork | 0.235 |
R-free | 0.25350 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2erk |
Data reduction software | XDS |
Data scaling software | Aimless (0.7.4) |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 47.120 | 47.120 | 2.550 |
High resolution limit [Å] | 2.450 | 8.830 | 2.450 |
Rmerge | 0.123 | 0.071 | 1.307 |
Rmeas | 0.131 | 0.075 | 1.395 |
Rpim | 0.045 | 0.025 | 0.478 |
Total number of observations | 140097 | 3105 | 15361 |
Number of reflections | 16460 | 374 | 1829 |
<I/σ(I)> | 10.1 | 28.7 | 1.5 |
Completeness [%] | 99.3 | 96.9 | 99 |
Redundancy | 8.5 | 8.3 | 8.4 |
CC(1/2) | 0.997 | 0.997 | 0.749 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.3 | 293 | 0.1M Tris pH 8.3, 20% PEG 8000 with 1.25 M NaCl in reservoir |