7OP0
Crystal structure of complement C5 in complex with chemically synthesized K92 knob domain.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-09-13 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 204.840, 104.820, 155.060 |
| Unit cell angles | 90.00, 125.13, 90.00 |
Refinement procedure
| Resolution | 56.670 - 2.570 |
| R-factor | 0.2329 |
| Rwork | 0.231 |
| R-free | 0.26420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7ad6 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.611 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.000 | 2.660 |
| High resolution limit [Å] | 2.570 | 2.570 |
| Number of reflections | 85364 | 8497 |
| <I/σ(I)> | 17.75 | 2.29 |
| Completeness [%] | 99.0 | |
| Redundancy | 13.4 | |
| CC(1/2) | 0.990 | 0.610 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.1 M bicine/Trizma (pH 8.5), 10 % (w/v) PEG 8000, 20 % (v/v) ethylene glycol, 30 mM sodium fluoride, 30 mM sodium bromide, 30 mM sodium iodide. |
