7OLI
Crystal structure of Pab-AGOG in complex with 8-oxoguanosine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-07-04 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.97857 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 131.063, 47.202, 91.271 |
Unit cell angles | 90.00, 100.80, 90.00 |
Refinement procedure
Resolution | 44.830 - 1.650 |
R-factor | 0.1628 |
Rwork | 0.161 |
R-free | 0.19070 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7olb |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.190 | 1.740 |
High resolution limit [Å] | 1.650 | 1.650 |
Rmerge | 0.081 | 0.404 |
Number of reflections | 65858 | 9640 |
<I/σ(I)> | 7.3 | 2.3 |
Completeness [%] | 99.3 | |
Redundancy | 3.2 | |
CC(1/2) | 0.992 | 0.837 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4 | 293 | LiCl, tri-Na citrate, PEG 6000 |