7OKP
Crystal structure of mouse CARM1 in complex with histone H3_13-22 K18 acetylated
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-X |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-03-24 |
Detector | DECTRIS EIGER R 4M |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 74.909, 99.580, 208.075 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.320 - 2.200 |
R-factor | 0.213 |
Rwork | 0.211 |
R-free | 0.25430 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5ih3 |
Data reduction software | XDS |
Data scaling software | Aimless (1.12.8) |
Phasing software | PHENIX (1.19.2_4158) |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.110 | 2.250 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.092 | 0.693 |
Rmeas | 0.101 | 0.809 |
Rpim | 0.040 | 0.406 |
Number of reflections | 77281 | 4317 |
<I/σ(I)> | 9.3 | 1.5 |
Completeness [%] | 97.1 | 93.7 |
Redundancy | 5.3 | 3.8 |
CC(1/2) | 0.998 | 0.690 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 1M sodium malonate, 0.1M MES pH7.0, 0.2M sodium chloride |