7OGS
X-ray Structure of Interferon Regulatory Factor 4 DNA binding domain bound to an interferon-stimulated response element
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-01-26 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9119 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 64.347, 66.082, 202.195 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 62.810 - 2.370 |
R-factor | 0.2183 |
Rwork | 0.217 |
R-free | 0.24940 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7o56 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.251 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | PHASER |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 64.350 | 2.410 |
High resolution limit [Å] | 2.370 | 2.370 |
Rmerge | 0.174 | 3.086 |
Rmeas | 0.189 | 3.409 |
Rpim | 0.074 | 1.434 |
Number of reflections | 35957 | 1740 |
<I/σ(I)> | 5.8 | 0.2 |
Completeness [%] | 100.0 | 100 |
Redundancy | 6.3 | 5.5 |
CC(1/2) | 0.998 | 0.281 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 0.1M Bis Tris pH 5.5, 0.2M ammonium acetate, 25% PEG 3350 |