7OGJ

Crystal structure of human METTL1 in complex with SAH

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-04-26
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	63.570, 80.460, 138.230
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.920 - 1.590
R-factor	0.1943
Rwork	0.193
R-free	0.21470
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ckk
RMSD bond length	0.007
RMSD bond angle	1.008
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.920	1.680
High resolution limit [Å]	1.590	1.590
Number of reflections	96318	15366
<I/σ(I)>	18.34
Completeness [%]	99.9
Redundancy	8.13
CC(1/2)	0.999	0.664

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277.15	200 mM lithium sulfate, 20% PEG 1000, 100 mM phosphate-citrate buffer pH 4.2