7O2I
METTL3-METTL14 heterodimer bound to the SAM competitive small molecule inhibitor STM2457
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-01-18 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 64.094, 64.094, 225.302 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.795 - 3.000 |
| Rwork | 0.186 |
| R-free | 0.26260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5l6d |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.287 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.795 | 3.180 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 1.800 | |
| Rmeas | 0.274 | |
| Number of reflections | 11480 | 1804 |
| <I/σ(I)> | 12.2 | 2.7 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 19.7 | 15 |
| CC(1/2) | 0.996 | 0.697 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 295 | 150 mM magnesium acetate, 22% w/v PEG 3350 |
