7O2F

Crystal structure of the human METTL3-METTL14 complex bound to Compound 22 (UZH2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-02-24
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1
Spacegroup name	P 32 2 1
Unit cell lengths	64.250, 64.250, 225.770
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.740 - 2.100
R-factor	0.1994
Rwork	0.197
R-free	0.24210
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7acd
RMSD bond length	0.008
RMSD bond angle	0.963
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.740	2.220
High resolution limit [Å]	2.100	2.100
Number of reflections	32606	5099
<I/σ(I)>	12.77
Completeness [%]	99.3
Redundancy	6.56
CC(1/2)	0.998	0.614

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	20% PEG 3350, 400mM Mg acetate