7O27
Crystal structure of the human METTL3-METTL14 complex bound to Compound 17 (ADO_AE_005)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-12-14 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 64.124, 64.124, 224.940 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.790 - 2.400 |
| R-factor | 0.2101 |
| Rwork | 0.208 |
| R-free | 0.25410 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7acd |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.080 |
| Data reduction software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.1_4122) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.800 | 2.550 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Number of reflections | 41982 | 3484 |
| <I/σ(I)> | 15.13 | |
| Completeness [%] | 99.9 | |
| Redundancy | 9.56 | |
| CC(1/2) | 0.999 | 0.415 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 295 | 20% PEG 3350, 400mM Mg acetate |
