7O27

Crystal structure of the human METTL3-METTL14 complex bound to Compound 17 (ADO_AE_005)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	N6-adenosine-methyltransferase catalytic subunit	polymer	246	28144.1	1	UniProt (Q86U44) Pfam (PF05063) In PDB	Homo sapiens (Human)	Methyltransferase-like protein 3,hMETTL3,N6-adenosine-methyltransferase 70 kDa subunit,MT-A70
2	B	N6-adenosine-methyltransferase non-catalytic subunit	polymer	290	33621.2	1	UniProt (Q9HCE5) Pfam (PF05063) In PDB	Homo sapiens (Human)	Methyltransferase-like protein 14,hMETTL14
3	A	4-[4-[(4,4-dimethylpiperidin-1-yl)methyl]phenyl]-9-(7H-pyrrolo[2,3-d]pyrimidin-4-yl)-1,4,9-triazaspiro[5.5]undecan-2-one	non-polymer		487.6	1	Chemie (UYZ)
4	B	ACETATE ION	non-polymer		59.0	1	Chemie (ACT)
5		water	water		18.0	38	Chemie (HOH)

Sequence modifications

A: 354 - 580 (UniProt: Q86U44)

PDB	External Database	Details
Met 335	-	initiating methionine
Gly 336	-	expression tag
His 337	-	expression tag
His 338	-	expression tag
His 339	-	expression tag
His 340	-	expression tag
His 341	-	expression tag
His 342	-	expression tag
Ser 343	-	expression tag
Ser 344	-	expression tag
Gly 345	-	expression tag
Arg 346	-	expression tag
Glu 347	-	expression tag
Asn 348	-	expression tag
Leu 349	-	expression tag
Tyr 350	-	expression tag
Phe 351	-	expression tag
Gln 352	-	expression tag
Gly 353	-	expression tag

B: 107 - 395 (UniProt: Q9HCE5)

PDB	External Database	Details
Met 106	-	initiating methionine

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	61765.3
Non-Polymers*	Number of molecules	2
	Total formula weight	546.7
All*	Total formula weight	62312.0

*Water molecules are not included.