7NZE
Crystal structure of HLA-DR4 in complex with a human collagen type II peptide
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-12-15 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9795 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 122.418, 71.840, 125.721 |
Unit cell angles | 90.00, 111.33, 90.00 |
Refinement procedure
Resolution | 47.009 - 2.050 |
Rwork | 0.192 |
R-free | 0.24490 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6bil |
RMSD bond length | 0.013 |
RMSD bond angle | 1.844 |
Data reduction software | REFMAC (5.8.0267) |
Data scaling software | Aimless |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 47.009 | 46.960 | 2.100 |
High resolution limit [Å] | 2.050 | 9.170 | 2.050 |
Number of reflections | 62583 | 4643 | 710 |
<I/σ(I)> | 8.2 | ||
Completeness [%] | 98.1 | ||
Redundancy | 1 | 1 | 1 |
CC(1/2) | 0.999 | 0.999 | 0.851 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 290 | 20% polyethylene glycol 3350 and 200 mM ammonium chloride |