7NZ9

Crystal structure of mouse ADAT2/ADAT3 tRNA deamination complex V128L mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-23
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.0
Spacegroup name	P 2 21 21
Unit cell lengths	51.976, 106.814, 129.668
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.380 - 1.990
R-factor	0.1904
Rwork	0.189
R-free	0.21780
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3dh1
RMSD bond length	0.007
RMSD bond angle	0.857
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.19rc4_4035)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.110
High resolution limit [Å]	1.990	1.990
Rmerge	0.136	2.440
Number of reflections	50047	7853
<I/σ(I)>	15.15	1.05
Completeness [%]	99.8
Redundancy	13.2
CC(1/2)	0.999	0.385

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	18% (W/V) PEG3350, 0.1 M Bis-Tris Propane pH 7.5 and 0.2 M NaBr