7NX3
Crystal structure of ALK in complex with Fab324
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-2 |
Synchrotron site | ESRF |
Beamline | ID23-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-03-01 |
Detector | DECTRIS PILATUS3 2M |
Wavelength(s) | 0.8731 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 109.700, 137.470, 144.260 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.040 - 2.810 |
R-factor | 0.2291 |
Rwork | 0.228 |
R-free | 0.26640 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5nuz |
RMSD bond length | 0.005 |
RMSD bond angle | 0.867 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.040 | 2.960 |
High resolution limit [Å] | 2.800 | 2.810 |
Rmeas | 0.250 | 1.200 |
Number of reflections | 55385 | 6835 |
<I/σ(I)> | 7.7 | |
Completeness [%] | 99.0 | |
Redundancy | 5.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 287 | 40mMPolyamines, 0.1M Gly-Gly/AMPD pH8.5 12.5% w/v PEG4000 20% w/v 1,2,6-Hexanetriol |