7NX0
LTK:ALKAL1 complex stabilized by a Nanobody
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 2 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-02-01 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.9800 |
Spacegroup name | P 61 |
Unit cell lengths | 129.110, 129.110, 109.750 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 42.260 - 1.950 |
R-factor | 0.1703 |
Rwork | 0.169 |
R-free | 0.19580 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7nx1 |
RMSD bond length | 0.005 |
RMSD bond angle | 0.899 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.300 | 2.000 |
High resolution limit [Å] | 1.950 | 1.950 |
Rmeas | 0.076 | 1.470 |
Number of reflections | 75541 | 5362 |
<I/σ(I)> | 26.6 | |
Completeness [%] | 99.9 | |
Redundancy | 20.8 | |
CC(1/2) | 0.990 | 0.880 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 287 | MOPSO/BIS-TRIS pH 6.5 20% PEG 8000 40% w/v 1,5-pentanediol 5mM Na2CrO4 . 4H2O 5mM Na2MoO4 . 4H2O 5mM Na2WO4 . 4H2O 5mM Na2VO4 . 4H2O |