7NNQ
Crystal structure of Mycobacterium tuberculosis ArgC in complex with nicotinamide adenine dinucleotide phosphate (NADP+)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-06-30 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.97625 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 69.050, 133.830, 83.050 |
Unit cell angles | 90.00, 109.39, 90.00 |
Refinement procedure
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 55.520 | 55.520 | 1.770 |
High resolution limit [Å] | 1.730 | 7.740 | 1.730 |
Rmerge | 0.056 | 0.029 | 1.461 |
Rmeas | 0.062 | 0.032 | 1.610 |
Rpim | 0.026 | 0.014 | 0.666 |
Total number of observations | 804727 | 9285 | 58410 |
Number of reflections | 145458 | 1698 | 10386 |
<I/σ(I)> | 13.9 | 47.4 | 1 |
Completeness [%] | 98.4 | 99.1 | 95.4 |
Redundancy | 5.5 | 5.5 | 5.6 |
CC(1/2) | 0.999 | 0.999 | 0.462 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.1 M Bis-Tris pH 7 17% PEG Smear High (PEGs 6K, 8K, 10K) 70 mM phosphate/citrate pH 5.6 20% glycerol |