7NHG

Crystal structure of the human METTL3-METTL14 complex with compound ASI_M3M_041

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-06-20
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1
Spacegroup name	P 32 2 1
Unit cell lengths	64.100, 64.100, 225.520
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.800 - 2.500
R-factor	0.2224
Rwork	0.220
R-free	0.27840
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5l6d
RMSD bond length	0.009
RMSD bond angle	0.984
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.800	2.650
High resolution limit [Å]	2.500	2.500
Number of reflections	19331	3065
<I/σ(I)>	9.93
Completeness [%]	99.1
Redundancy	6.31
CC(1/2)	0.993	0.478

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	20% PEG 3350, 400mM Mg acetate