7NDY
Di-phosphorylated Barrier-to-Autointegration Factor (BAF) in complex with LEM domain of Emerin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2020-11-25 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 0.87313 |
| Spacegroup name | P 1 |
| Unit cell lengths | 33.050, 40.628, 43.224 |
| Unit cell angles | 69.51, 69.68, 85.03 |
Refinement procedure
| Resolution | 38.030 - 1.440 |
| R-factor | 0.1829 |
| Rwork | 0.181 |
| R-free | 0.22500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6ghd |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.870 |
| Data reduction software | XDS |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.225 | 1.411 |
| High resolution limit [Å] | 1.242 | 1.242 |
| Rmerge | 0.802 | |
| Rmeas | 0.965 | |
| Rpim | 0.527 | |
| Number of reflections | 32449 | 1622 |
| <I/σ(I)> | 4.5 | 1.3 |
| Completeness [%] | 84.8 | |
| Redundancy | 15 | |
| CC(1/2) | 0.989 | 0.326 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | Hepes Sodium salt 0.1 M pH 7.5 PEG 600 25% W/V |
