7N94

LINE-1 endonuclease domain complex with DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	93
Detector technology	PIXEL
Collection date	2020-03-20
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.03
Spacegroup name	P 43 21 2
Unit cell lengths	100.774, 100.774, 140.770
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.920 - 2.850
R-factor	0.2209
Rwork	0.214
R-free	0.28030
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1vyb
RMSD bond length	0.008
RMSD bond angle	1.263
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.900
High resolution limit [Å]	2.850	2.850
Rmerge	0.063	1.750
Rpim	0.017	0.464
Number of reflections	17800	888
<I/σ(I)>	40.5
Completeness [%]	100.0	100
Redundancy	14.7	14.8
CC(1/2)	0.983	0.759

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	6	293	0.1M MES pH 6.0, 0.2M NaCl, 30% Jeffamine ED-2003