7N8Q

Rhesusized RV305 DH677.3 Fab bound to Clade A/E 93TH057 HIV-1 gp120 core.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-29
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97946
Spacegroup name	P 1 21 1
Unit cell lengths	99.182, 82.664, 111.899
Unit cell angles	90.00, 112.02, 90.00

Refinement procedure

Resolution	47.930 - 2.900
R-factor	0.2572
Rwork	0.255
R-free	0.29578
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6mfp
RMSD bond length	0.010
RMSD bond angle	1.720
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.950
High resolution limit [Å]	2.900	2.900
Rmerge	0.106	0.627
Rpim	0.078	0.467
Number of reflections	31091	1645
<I/σ(I)>	9.6	1.1
Completeness [%]	82.7	86
Redundancy	2.5	2.5
CC(1/2)	0.975	0.714

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	294	20% PEG 4000 0.1 M sodium citrate pH 4.5